Data to be generated

Project 1	Data Generation
Brain tissue from H1 and H2 with European & African ancestry
	single nuclei multi-Omic (ATAC, RNAseq), ISOseq
	proteomics
	immunohistochemistry
iPSC neuron, astrocyte, microglia from H1 and H2
	bulk RNAseq, ATACseq, ISOseq, Hi-C
	proteomics
iPSC assembloids from H1 and H2
	single nuclei multi-Omic (ATAC, RNAseq)
	proteomics
iPSC assembloid with CRISPR of candidate regulatory elements in H1 vs H2	single nuclei RNAseq

Project 2
Brain tissue from FTD mutation
	single nuclei multi-Omic (ATAC, RNAseq), ISOseq
	proteomics
	immunohistochemistry
iPSC assembloid with CRISPR of MAPT mutations on H1 and H2
	iPSC assembloid with CRISPR of MAPT mutations on H1 and H2 single nuclei multi-Omic (ATAC, RNAseq), ISOseq
	proteomics
iPSC assembloid with CRISPR candidate regulatory elements on H1 and H2
	single nuclei RNAseq
	proteomics
	immunohistochemistry
	cellular phenotypes